Pixelgen Proxiome Kit v2

The Pixelgen Proxiome Kit v2 - Mechanistic understanding of cell function through protein interactomics

The Pixelgen Proxiome Kit v2 enables high-dimensional analysis of cell surface architecture in single human immune cells, generating readouts on protein abundance, protein clustering, and protein colocalization for 155 surface proteins. It is provided as a sample prep kit enabling direct implementation in any lab, without the need for acquiring expensive specialized equipment. The kit is available in 8-, 16-, and 32-reaction formats, supporting up to 64 samples per experiment, and can be combined with the Pixelgen Proxiome Custom Conjugation and Pixelgen Proxiome CAR T products for increased customization. 

• Research areas: hematology, CAR-T / cell therapy, immuno-oncology, autoimmunity, infectious disease, fundamental immunology and more

• Readout: Cell surface architecture analysis of human immune cells

• Data output: Single cell proximity networks with associated abundance, clustering and colocalization scores for each cell

• Resolution: Nanoscale (approximately ~50 nm)

• Species: Human

• Multiplex: 155 cell surface proteins

• Add-ons: Support for up to 2 custom antibodies using the Pixelgen Proxiome v2 Custom Conjugation Kit and up to 2 CAR-specific detection reagents through the Pixelgen Proxiome CAR T products 

• Throughput: Up to 64 samples per experiment

• Cell input: from 50 000 cells per sample

• Cell output: 1000 cells per sample

• Supported sample input: Cells in solution originating from live or frozen PBMCs, bone marrow, synovial fluid, isolated immune cells, cell lines, cells dissociated from organoids or fresh frozen tissue etc. 

• Workflow: Standard molecular biology workflow with sequencing support for Illumina, MGI, Roche, Ultima Genomics, and AVITI platforms

• Analysis: Pixelator software for data processing, spatial analysis, and integration with standard single-cell analysis workflows