Pixelgen Proxiome Kit

The Pixelgen Proxiome Kit, Immuno 155 offers a unique ability to map the protein interactome of single cells.

The Pixelgen Proxiome Kit is based on the Proximity Network Assay, a technology for the nanoscale spatial analysis of immune cell proteins. Barcoded antibodies are bound to cells in suspension and in situ amplified by rolling circle amplification (RCA). RCA is followed by the addition of proximity oligos and a gap fill-ligation reaction to form multiple connections between neighboring proteins. The data generated after sequencing these molecules create a nanoscale protein Proximity Network for each cell, which is then analyzed using spatial statistics to define the organization of each cell's protein interactome, as well as cell-cell interactions.

The NGS data output from 1000 cells per sample is processed using the Pixelator primary analysis tool and analysis packages and provides a digital 3D representation of the cell surface proteins together with protein abundance, protein clustering, and protein colocalization. The pre-validated immunology panel consists of 155 protein assay targets, including 4 internal controls.

Order your kit today and enable a mechanistic understanding of cell function by protein interactomics.